Bioinformatics analysis of the wheel treadmill test on motor function recovery after spinal cord injury

This study aimed to explore the possible target and mechanism of wheel treadmill (WTM) test for motor function recovery spinal cord injury (SCI). Rats were divided into sham, control WTM groups establish an SCI mode. in group trained on test, Basso–Beattie–Bresnahan (BBB) scores determined. The samples collected, mRNA sequencing was conducted determine changes gene expression. coexpressed genes screened construct a protein–protein interaction (PPI), followed by Kyoto Encyclopedia Genes Genomes pathway Gene Ontology enrichment analysis, differentially expressed (DEGs) volcano map hub expression heat constructed using R language. BBB increased with time, scoring higher than group. results rat tissue showed that total 1679 DEGs sham groups; 928 731 overlapping groups. key identified PPI analysis. One hundred thirty-three found be combined analysis data rats at Week 7. top 10 from high low Tyrobp, Rac2, Cd68, C1qb, Aif1, Cd74, Spi1, Fcer1g, RT1-DA, Ccl4. terms highest microglia-mediated positive regulation cytotoxicity major histocompatibility complex class II protein complexes. Treatment promotes after modulating intercellular communication immune function.